Oxidative DNA damage / Gentotoxicity

Oxidative damage to nucleic acids is implicated in a wide range of pathological conditions such as cancer, inflammation, and many degenerative diseases. Furthermore, genotoxicity is an important consideration in the testing of potential pharmaceuticals. Damage is mediated via free radicals that can be created by a range of agents such as xenobiotics, radiation, ischaemia-reperfusion injury and normal metabolic activity. These free radicals may react with DNA causing reversible and irreversible damage, leading to mutation, carcinogenesis or cell death. 8-oxoguanine is the most common DNA adduct formed during oxidative DNA damage and its production is linked to an increased risk of mutagenesis

The OxyDNA test can be used to detect oxidative DNA damage in situ in cell cultures affixed to slides and in cell suspensions using the FACS technique. The Biotrin OxyDNA Assay provides a simple, convenient and sensitive fluorescence method for the study of oxidative DNA damage

The ease of use and rapid results obtained with the Biotrin OxyDNA Test offer the potential for studying oxidative DNA damage in a wide range of medical conditions plus simplifying the in-vitro testing of potential pharmaceuticals for genotoxicity. 8-oxoguanine is the clinically most significant form of oxidative DNA damage, frequently leading to mutagenesis or carcinogenesis.

Kit insert	MSDS	OxyDNA flyer	OxyDNA info