PI GST EIA

INTENDED USE

Pi glutathione S-transferase (pi GST) is found in distal regions of the human nephron. In the liver, it is found
in the biliary epithelium. Urinary pi GST is a sensitive and specific biomarkers for renal distal tubular injury.
Elevated plasma pi GST is found in a variety of liver diseases and in some malignancies.

ASSAY PRINCIPLE

The Biotrin Pi GST EIA is a quantitative enzyme immunoassay for the assay of pi GST in human plasma and
urine. For the assay of pi GST in other materials, contact Biotrin for advice.

The test procedure is based on the sequential addition of sample, anti pi GST -enzyme conjugate, and
substrate to microassay wells coated with anti pi GST. The resultant colour intensity is proportional to the
amount of pi GST present in the sample. The assay range is 3-100 microg/L, equivalent to 7.5-250 microg/L
in stabilised urine samples diluted 1/2, or 15-500 microg/L in plasma samples diluted 1/5. Total assay time is
2½ hours.

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