**United States Sales and Usage Limitation: For Research Use Only. Not for use in diagnostic procedures.

INTENDED USE

The Biotrin Human Herpesvirus-6 IgM IFA is intended for the qualitative and semi-quantitative detection of anti-Human Herpesvirus-6 (HHV-6) IgM antibodies in serum and plasma.

ASSAY PRINCIPLE

The Biotrin HHV-6 IFA system utilises the indirect immunofluorescent method of antibody detection and titre determination.

Patient samples are incubated with HHV-6 antigen, which has been stabilised on a glass slide. If HHV-6 IgM antibodies are present in the sample, a stable complex is formed between the antibody and the antigen on the slide.

Bound HHV 6 IgM antibody is then reacted with a fluorescein conjugated goat anti-human IgG and this complex is visualised with the aid of a fluorescence microscope.

Positive reactions are denoted by bright green fluorescence of infected cells. Uninfected cells are stained red due to the used of a counter stains

  Rapid easy to use assay

  High levels of cells in each well

 Good representation of HHV-6 positive and negative cells in each well

  Positive and Negative controls included